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IRE1 Rabbit pAb (A17940)

Publications (20) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IRE1 Rabbit pAb (A17940)

Western blot analysis of lysates from 293T cells, using IRE1 Rabbit pAb (A17940) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

ABclonal:Western blot - IRE1 Rabbit pAb (A17940)

Western blot analysis of various lysates using IRE1 Rabbit pAb (A17940) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - IRE1 Rabbit pAb (A17940)

Immunofluorescence analysis of U2OS cells using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IRE1 Rabbit pAb (A17940)

Immunofluorescence analysis of NIH/3T3 cells using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IRE1 Rabbit pAb (A17940)

Immunofluorescence analysis of PC-12 cells using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIRE1 Rabbit pAb
Catalog No.A17940
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 286-435 of human IRE1 (NP_001424.3).
SequenceVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEA
Gene ID
Swiss Prot
SynonymsIRE1; IRE1P; IRE1a; hIRE1p
Calculated MW110kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Mouse liver, Rat liver
Cellular locationEndoplasmic reticulum membrane, Single-pass type I membrane protein.
Customer validation

WB(Rattus norvegicus, Homo sapiens, Chlorocebus aethiops, Mus musculus, Anatinae)

IF(Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - IRE1 Rabbit pAb (A17940)}

    Western blot - IRE1 Rabbit pAb (A17940)

    Western blot analysis of lysates from 293T cells, using IRE1 Rabbit pAb (A17940) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.
    ABclonal:Western blot - IRE1 Rabbit pAb (A17940)}

    Western blot - IRE1 Rabbit pAb (A17940)

    Western blot analysis of various lysates using IRE1 Rabbit pAb (A17940) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)}

    Immunohistochemistry - IRE1 Rabbit pAb (A17940)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)}

    Immunohistochemistry - IRE1 Rabbit pAb (A17940)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)}

    Immunohistochemistry - IRE1 Rabbit pAb (A17940)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)}

    Immunohistochemistry - IRE1 Rabbit pAb (A17940)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - IRE1 Rabbit pAb (A17940)}

    Immunohistochemistry - IRE1 Rabbit pAb (A17940)

    Immunohistochemistry analysis of paraffin-embedded Rat pancreas using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - IRE1 Rabbit pAb (A17940)}

    Immunofluorescence - IRE1 Rabbit pAb (A17940)

    Immunofluorescence analysis of U2OS cells using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - IRE1 Rabbit pAb (A17940)}

    Immunofluorescence - IRE1 Rabbit pAb (A17940)

    Immunofluorescence analysis of NIH/3T3 cells using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - IRE1 Rabbit pAb (A17940)}

    Immunofluorescence - IRE1 Rabbit pAb (A17940)

    Immunofluorescence analysis of PC-12 cells using IRE1 Rabbit pAb (A17940) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (3)

    Secondary Antibodies (26)