Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:3000 dilution. HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of lysates from 293T cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. 293T cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from 293T cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from C6 cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from C6 cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631).NIH-3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb |
|---|---|
| Catalog No. | AP0631 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0193 |
| Immunogen | A synthetic phosphorylated peptide around T183 of human JNK1 (P45983). |
|---|---|
| Sequence | MMTPY |
| Gene ID | |
| Swiss Prot | |
| Synonyms | JNK1/JNK2/JNK3; Phospho-JNK1/2/3-T183/T183/T221 |
| Calculated MW | 35kDa/44kDa/48kDa/27kDa/52kDa |
| Observed MW | 46kDa/54kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HeLa treated by Anisomycin, 293T treated by Anisomycin, 293T treated by UV, C6 treated by UV, C6 treated by Anisomycin |
| Cellular location | axon, basal dendrite, cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus, synapse |
| Customer validation | WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Capra hircus, Bos taurus, Oryza sativa, Chlorocebus aethiops, Mus musculus, Rattus norvegicus, Chrysanthemum, Bos taurus, Sus scrofa) IHC(Mus musculus) IF(Rattus norvegicus) IHC(Chrysanthemum) |
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