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Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Publications (64) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of lysates from HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:3000 dilution. HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of lysates from C6 cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of lysates from C6 cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631).NIH-3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-JNK1/2/3-T183/T183/T221 Rabbit mAb
Catalog No.AP0631
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0193
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
ImmunogenA synthetic phosphorylated peptide around T183 of human JNK1 (P45983).
SequenceMMTPY
Gene ID
Swiss Prot
SynonymsJNK1/JNK2/JNK3; Phospho-JNK1/2/3-T183/T183/T221
Calculated MW35kDa/44kDa/48kDa/27kDa/52kDa
Observed MW46kDa/54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa treated by Anisomycin, 293T treated by Anisomycin, 293T treated by UV, C6 treated by UV, C6 treated by Anisomycin
Cellular locationaxon, basal dendrite, cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus, synapse.
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Capra hircus, Bos taurus, Oryza sativa, Chlorocebus aethiops, Mus musculus, Rattus norvegicus, Chrysanthemum, Bos taurus, Sus scrofa)

IHC(Mus musculus)

IF(Rattus norvegicus)

IHC(Chrysanthemum)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Western blot analysis of lysates from HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:3000 dilution. HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Western blot analysis of lysates from C6 cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Western blot analysis of lysates from C6 cells, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (A23099) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

    Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631).NIH-3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0631? Please let us know so that we can cite the reference in this datasheet.

    항체 (4)

    JNK1/2/3 Rabbit mAb
    A4867

    WBIF/ICCELISA

    Reactivity:Human, Mouse, Rat

    JNK1/2/3 Rabbit mAb

    JNK1/2/3 Rabbit pAb
    A18678

    ELISAWBIHC-PIF/ICCIP

    Reactivity:Human, Mouse, Rat

    JNK1/2/3 Rabbit pAb

    Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb
    AP1163

    ELISAWB

    Reactivity:Human, Mouse

    Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit pAb

    JNK1/2/3 Rabbit mAb
    A22376

    WBDBIF/ICCELISA

    Reactivity:Human, Mouse, Rat

    JNK1/2/3 Rabbit mAb

    Secondary Antibodies (25)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb