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JNK1/2/3 Rabbit pAb (A18678)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using JNK1/2/3 Rabbit pAb (A18678) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - JNK1/2/3 Rabbit pAb (A18678)

Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 Rabbit pAb (A18678) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 Rabbit pAb (A18678) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 Rabbit pAb (A18678) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat brain using JNK1/2/3 Rabbit pAb (A18678) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - JNK1/2/3 Rabbit pAb (A18678)

Immunofluorescence analysis of U2OS cells using JNK1/2/3 Rabbit pAb (A18678) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - JNK1/2/3 Rabbit pAb (A18678)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain cells using 3 μg JNK1/2/3 antibody (A18678). Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody (A18678) at a dilution of 1:1000.

Overview

Product name	JNK1/2/3 Rabbit pAb
Catalog No.	A18678
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

JNK belongs to MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1, JNK2, and JNK3

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human JNK1/2/3 (NP_620637.1).
Sequence	MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNG
Gene ID
Swiss Prot
Synonyms
Calculated MW	35kDa/44kDa/48kDa/27kDa/52kDa
Observed MW	46kDa/54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	SH-SY5Y, Mouse brain, Rat brain
Cellular location	axon, basal dendrite, cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus, synapse
Customer validation	WB(Bos taurus, Other, Homo sapiens)