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Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from RAW264.7 cells, using Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530) at 1:1000 dilution. RAW264. 7 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-Jak1-Y1034/Y1035 Rabbit pAb
Catalog No.	AP0530
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta, interferon-gamma, and cytokine signal transduction. This gene plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. This gene is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune and inflammation response and is a therapeutic target for alleviating cytokine storms. The kinase activity of this gene is directly inhibited by the suppressor of cytokine signalling 1 (SOCS1) protein. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y1034 & Y1035 of human JAK1 (NP_002218.2).
Sequence	KEYYTV
Gene ID
Swiss Prot
Synonyms	JTK3; AIIDE; JAK1A; JAK1B; Phospho-Jak1-Y1034/1035
Calculated MW	133kDa
Observed MW	130kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	RAW264.7 treated by Pervanadate
Cellular location	Endomembrane system, Peripheral membrane protein.
Customer validation	WB(Rattus norvegicus, Homo sapiens, Mus musculus, Mus musculus)

Documents

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Research Area

Phospho-Jak1-Y1034/Y1035 Rabbit pAb images

Western blot - Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Immunofluorescence - Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

3 Biotech

Application:

IF:

2.6

Species:

Rattus norvegicus

PMID:

34549015

Title:

Majoon Chobchini attenuates arthritis disease severity and RANKL-mediated osteoclastogenesis in rheumatoid arthritis

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

34867333

Title:

VDR Signaling via the Enzyme NAT2 Inhibits Colorectal Cancer Progression

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Brain, behavior, and immunity

Application:

IF:

8.8

Species:

Rattus norvegicus

PMID:

33486004

Title:

DHA attenuated Japanese Encephalitis virus infection-induced neuroinflammation and neuronal cell death in cultured rat Neuron/glia

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

ACS nano

Application:

IF:

15.8

Species:

Mus musculus

PMID:

35005890

Title:

Biomimetic Hydroxyapatite Nanorods Promote Bone Regeneration via Accelerating Osteogenesis of BMSCs through T Cell-Derived IL-22

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Cells

Application:

IF:

7.7

Species:

Mus musculus

PMID:

36429017

Title:

Abrocitinib Attenuates Microglia-Mediated Neuroinflammation after Traumatic Brain Injury via Inhibiting the JAK1/STAT1/NF-κB Pathway - PMC

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

37744350

Title:

TAGAP expression influences CD4+ T cell differentiation, immune infiltration, and cytotoxicity in LUAD through the STAT pathway: implications for immunotherapy - PMC

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.6

Species:

Homo sapiens, Mus musculus

PMID:

36838711

Title:

Centipeda minima Extract Inhibits Inflammation and Cell Proliferation by Regulating JAK/STAT Signaling in Macrophages and Keratinocytes - PMC

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

37977740

Title:

(E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol alleviates inflammatory responses in LPS-induced mice liver sepsis through inhibition of STAT3

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36791928

Title:

Panlongqi tablet suppresses adjuvant-induced rheumatoid arthritis by inhibiting the inflammatory reponse in vivo and in vitro

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Cancer gene therapy

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

37990063

Title:

Cell fusion upregulates PD-L1 expression for evasion from immunosurveillance

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Chinese medicine

Application:

IF:

5.3

Species:

Homo sapiens

PMID:

38360696

Title:

Paclitaxel combined with Compound K inducing pyroptosis of non-small cell lung cancer cells by regulating Treg/Th17 balance - PMC

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

International journal of oncology

Application:

IF:

4.5

Species:

Homo sapiens

PMID:

35059729

Title:

Extracellular domain of semaphorin 5A serves a tumorsuppressing role by activating interferon signaling pathways in lung adenocarcinoma cells

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38310765

Title:

Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Mus musculus

PMID:

37400369

Title:

LiverInspired Polyetherketoneketone Scaffolds Simulate Regenerative Signals and Mobilize AntiInflammatory Reserves to Reprogram Macrophage Metabolism for

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.7

Species:

Mus musculus

PMID:

38539789

Title:

Transcriptotype-Driven Discovery of Apigenin as a Therapy against Cholestatic Liver Fibrosis: Through Inhibition of PANoptosis and Following Type-I

References for Phospho-Jak1-Y1034/Y1035 Rabbit pAb (AP0530)

Product:

Phospho-Jak1-Y1034/Y1035 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Rattus norvegicus

PMID:

38810305

Title:

IL-17A/IL-17RA interaction blockade sensitizes synovial macrophages to efferocytosis and PD-L1 signaling via rewiring STAT-3/ADAM17/MERTK axis in rheumatoid