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Phospho-KAP1/TRIM28-S824 Rabbit mAb (AP1393)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from HeLa cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb (AP1393) at 1:5000 dilution. HeLa cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb (AP1393) at 1:5000 dilution. NIH/3T3 cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name	Phospho-KAP1/TRIM28-S824 Rabbit mAb
Catalog No.	AP1393
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59356

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S824 of human KAP1/TRIM28 (NP_005753.1 ).
Sequence	LSSQE
Gene ID
Swiss Prot
Synonyms	KAP1; TF1B; RNF96; TIF1B; PPP1R157; TIF1beta; Phospho-KAP1/TRIM28-S824
Calculated MW	79kDa/89kDa
Observed MW	117kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Etoposide, NIH/3T3 treated by Etoposide
Cellular location	Nucleus