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Phospho-LIMK2-T505 Rabbit pAb (AP0388)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-LIMK2-T505 Rabbit pAb (AP0388)

Western blot analysis of lysates from HeLa cells, using Phospho-LIMK2-T505 Rabbit pAb (AP0388).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-LIMK2-T505 Rabbit pAb
Catalog No.AP0388
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding T505 of human LIMK2
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsLIMK2; Phospho-LIMK2-T505
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus, Nucleus
ABclonal:Western blot - Phospho-LIMK2-T505 Rabbit pAb (AP0388)}

Western blot - Phospho-LIMK2-T505 Rabbit pAb (AP0388)

Western blot analysis of lysates from HeLa cells, using Phospho-LIMK2-T505 Rabbit pAb (AP0388).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)