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Phospho-LRP6-S1490 Rabbit pAb (AP1191)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-LRP6-S1490 Rabbit pAb (AP1191)

Western blot analysis of lysates from HeLa cells, using Phospho-LRP6-S1490 Rabbit pAb (AP1191) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product namePhospho-LRP6-S1490 Rabbit pAb
Catalog No.AP1191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.
ImmunogenA synthetic phosphorylated peptide around S1490 of human LRP6 (NP_002327.2).
SequencePPSPA
Gene ID
Swiss Prot
SynonymsADCAD2; STHAG7; Phospho-LRP6-S1490
Calculated MW180kDa
Observed MW180kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by Calyculin A
Cellular locationEndoplasmic reticulum, Membrane, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - Phospho-LRP6-S1490 Rabbit pAb (AP1191)}

Western blot - Phospho-LRP6-S1490 Rabbit pAb (AP1191)

Western blot analysis of lysates from HeLa cells, using Phospho-LRP6-S1490 Rabbit pAb (AP1191) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)