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Phospho-LRRK2-S935 Rabbit mAb (AP1154)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LRRK2-S935 Rabbit mAb (AP1154) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Western blot analysis of lysates from C6 cells, using Phospho-LRRK2-S935 Rabbit mAb (AP1154) at 1:1000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product name	Phospho-LRRK2-S935 Rabbit mAb
Catalog No.	AP1154
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1694

Background

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S935 of human LRRK2 (Q5S007).
Sequence	SNSLG
Gene ID
Swiss Prot
Synonyms	PARK8; RIPK7; ROCO2; AURA17; DARDARIN; Phospho-LRRK2-S935
Calculated MW	286kDa
Observed MW	286kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by Calyculin A, C6 treated by Calyculin A
Cellular location	Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, Mitochondrion outer membrane, Perikaryon, Peripheral membrane protein, axon, dendrite, secretory vesicle, synaptic vesicle membrane.