Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y396 Rabbit mAb (AP1050) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
| Product name | Phospho-Lyn-Y396 Rabbit mAb |
|---|---|
| Catalog No. | AP1050 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1597 |
| Immunogen | A synthetic phosphorylated peptide around Y396 of human Lyn (P07948). |
|---|---|
| Sequence | DNEYT |
| Gene ID | |
| Swiss Prot | |
| Synonyms | JTK8; p53Lyn; p56Lyn; Phospho-Lyn-Y396 |
| Calculated MW | 56kDa/59kDa |
| Observed MW | 56kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | NIH/3T3 treated by pervanadate |
| Cellular location | Cell membrane, Cytoplasm, perinuclear region, Golgi apparatus, Membrane, Nucleus. |
| Customer validation | WB(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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