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Phospho-MARCKS-S162 Rabbit pAb (AP0403)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MARCKS-S162 Rabbit pAb (AP0403)

Western blot analysis of lysates from 293T cells, using Phospho-MARCKS-S162 Rabbit pAb (AP0403) at 1:400 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-MARCKS-S162 Rabbit pAb
Catalog No.AP0403
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
ImmunogenA phospho specific peptide corresponding to residues surrounding S162 of human MARCKS
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsMACS; 80K-L; PKCSL; PRKCSL; Phospho-MARCKS-S162
Calculated MW32kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by PMA
Cellular locationCytoplasm, Lipid-anchor, Membrane, cytoskeleton.

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ABclonal:Western blot - Phospho-MARCKS-S162 Rabbit pAb (AP0403)}

Western blot - Phospho-MARCKS-S162 Rabbit pAb (AP0403)

Western blot analysis of lysates from 293T cells, using Phospho-MARCKS-S162 Rabbit pAb (AP0403) at 1:400 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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