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Phospho-MEK2-T394 Rabbit pAb (AP0121)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-MEK2-T394 Rabbit pAb (AP0121)

Western blot analysis of lysates from HepG2 cells using Phospho-MEK2-T394 Rabbit pAb (AP0121).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-MEK2-T394 Rabbit pAb
Catalog No.AP0121
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding T394 of human MEK2
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsCFC4; MEK2; MKK2; MAPKK2; PRKMK2; Phospho-MEK2-T394
Calculated MW44kDa
Observed MW44kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2
Cellular locationCytoplasm, Membrane, Peripheral membrane protein
Customer validation

WB(Homo sapiens)

Co-IP(Homo sapiens,Mus musculus)

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - Phospho-MEK2-T394 Rabbit pAb (AP0121)}

Western blot - Phospho-MEK2-T394 Rabbit pAb (AP0121)

Western blot analysis of lysates from HepG2 cells using Phospho-MEK2-T394 Rabbit pAb (AP0121).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0121? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)