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Phospho-MYH9-S1943 Rabbit pAb (AP0802)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MYH9-S1943 Rabbit pAb (AP0802)

Western blot analysis of lysates from 293T cells, using Phospho-MYH9-S1943 Rabbit pAb (AP0802) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product namePhospho-MYH9-S1943 Rabbit pAb
Catalog No.AP0802
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
ImmunogenA synthetic phosphorylated peptide around S1943 of human MYH9 (NP_002464.1).
SequenceDGSDE
Gene ID
Swiss Prot
SynonymsMHA; FTNS; EPSTS; BDPLT6; DFNA17; MATINS; NMMHCA; NMHC-II-A; NMMHC-IIA; Phospho-MYH9-S1943
Calculated MW227kDa
Observed MW250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationCytoplasm, cell cortex, cytoskeleton
Customer validation

IF(Homo sapiens)

WB(Homo sapiens)

ABclonal:Western blot - Phospho-MYH9-S1943 Rabbit pAb (AP0802)}

Western blot - Phospho-MYH9-S1943 Rabbit pAb (AP0802)

Western blot analysis of lysates from 293T cells, using Phospho-MYH9-S1943 Rabbit pAb (AP0802) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

ELISA 키트 (1)

Secondary Antibodies (26)