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Phospho-Met-Y1234/Y1235 Rabbit mAb (AP1339)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 Rabbit mAb (AP1339) at1:2000 dilution. HeLa cells were treated by HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 Rabbit mAb (AP1339) at1:2000 dilution. C2C12 and C6 cells were treated by HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product name	Phospho-Met-Y1234/Y1235 Rabbit mAb
Catalog No.	AP1339
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC54652

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y1234 & Y1235 of human Met (NP_000236.2).
Sequence	KEYYSV
Gene ID
Swiss Prot
Synonyms	DA11; HGFR; AUTS9; RCCP2; c-Met; DFNB97; Phospho-Met-Y1234/Y1235
Calculated MW	156kDa
Observed MW	145kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by HGF, C2C12 treated by HGF, C6 treated by HGF
Cellular location	basal plasma membrane, cell surface, extracellular region, plasma membrane.
Customer validation	WB(Mus musculus)