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Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using Phospho-NF-κB p105-S932 Rabbit mAb (AP1355) at1:2000 dilution. HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates, using Phospho-NF-κB p105-S932 Rabbit mAb (AP1355) at1:2000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	Phospho-NF-κB p105-S932 Rabbit mAb
Catalog No.	AP1355
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55043

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S932 of human Phospho-NF-κB p105-S932 (NP_001158884.1).
Sequence	ETSFR
Gene ID
Swiss Prot
Synonyms	KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta; Phospho-NF-κB p105-S932
Calculated MW	105kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by TNF-α and Calyculin A, NIH/3T3 treated by TNF-α and Calyculin A
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Homo sapiens) IF(Homo sapiens) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-NF-κB p105-S932 Rabbit mAb images

Western blot - Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

PLoS neglected tropical diseases

Application:

IF:

4.78

Species:

Mus musculus

PMID:

35482821

Title:

COX-2 is required to mediate crosstalk of ROS-dependent activation of MAPK/NF-κB signaling with pro-inflammatory response and defense-related NO enhancement during challenge of macrophage-like cell line with Giardia duodenalis

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

36361656

Title:

Effects of Sarcoptes scabiei Translationally Controlled Tumor Protein (TCTP) on Histamine Release and Degranulation of KU812 Cells - PMC

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

European journal of medicinal chemistry

Application:

IF:

7.08

Species:

Mus musculus

PMID:

37137246

Title:

Design, synthesis, and SAR study of novel flavone 1, 2, 4-oxadiazole derivatives with anti-inflammatory activities for the treatment of Parkinson's disease

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Cellular signalling

Application:

IF:

4.85

Species:

Mus musculus

PMID:

37075875

Title:

Programmed cell death 10 increased blood-brain barrier permeability through HMGB1/TLR4 mediated downregulation of endothelial ZO-1 in glioblastoma

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Science advances

Application:

IF:

14.95

Species:

Mus musculus

PMID:

37851811

Title:

Neurological disorders after severe pneumonia are associated with translocation of endogenous bacteria from the lung to the brain - PMC

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Journal of controlled release : official journal of the Controlled Release Society

Application:

IF:

11.46

Species:

Mus musculus

PMID:

37619863

Title:

Surface-tethered ROS-responsive micelle backpacks for boosting mesenchymal stem cell vitality and modulating inflammation in ischemic stroke treatment

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

WB, IF

IF:

7.41

Species:

Homo sapiens

PMID:

37913735

Title:

SHP2 potentiates anti-PD-1 effectiveness through intervening cell pyroptosis resistance in triple-negative breast cancer

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

17.52

Species:

Mus musculus

PMID:

37400369

Title:

LiverInspired Polyetherketoneketone Scaffolds Simulate Regenerative Signals and Mobilize AntiInflammatory Reserves to Reprogram Macrophage Metabolism for

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38834834

Title:

Bacteroidetes promotes esophageal squamous carcinoma invasion and metastasis through LPS-mediated TLR4/Myd88/NF-B pathway and inflammatory changes - PMC

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

38705987

Title:

Irradiated microparticles suppress prostate cancer by tumor microenvironment reprogramming and ferroptosis - PMC

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Biochemical genetics

Application:

IF:

2.4

Species:

Homo sapiens

PMID:

38478147

Title:

DR6 Augments Colorectal Cancer Cell Growth, Invasion, and Stemness by Activating AKT/NF-B Pathway

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Toxicology and applied pharmacology

Application:

IF:

3.8

Species:

Mus musculus

PMID:

38797265

Title:

PI3K inhibitor "alpelisib" alleviates methotrexate induced liver injury in mice and potentiates its cytotoxic effect against MDA-MB-231 triple negative breast cancer cell

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Journal of translational medicine

Application:

IF:

7.4

Species:

Mus musculus

PMID:

38840216

Title:

S1PR3 inhibition protects against LPS-induced ARDS by inhibiting NF-B and improving mitochondrial oxidative phosphorylation - PMC

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.3

Species:

Mus musculus

PMID:

39068433

Title:

Nanoligomers targeting NF-B and NLRP3 reduce neuroinflammation and improve cognitive function with aging and tauopathy - PMC

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

Biotechnology and applied biochemistry

Application:

IHC

IF:

2.8

Species:

Mus musculus

PMID:

38798098

Title:

The inhibitory impact of Schisandrin on inflammation and oxidative stress alleviates LPSinduced acute kidney injury

References for Phospho-NF-κB p105-S932 Rabbit mAb (AP1355)

Product:

Phospho-NF-κB p105-S932 Rabbit mAb

Journal:

International wound journal

Application:

IF:

3.1

Species:

Homo sapiens

PMID:

39379061

Title:

Combination therapy of negative pressure wound therapy and antibiotic-loaded bone cement for accelerating diabetic foot ulcer healing: A prospective randomised controlled trial - PMC