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Phospho-IRS1-S612/S636 Rabbit mAb (AP1507)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from C2C12 cells, using Phospho-IRS1-S612/S636 Rabbit mAb (AP1507) at 1:1000 dilution.C2C12 cells were treated by Insulin (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-IRS1-S612/S636 Rabbit mAb (AP1507)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-IRS1-S612/S636 Rabbit mAb (AP1507) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	Phospho-IRS1-S612/S636 Rabbit mAb
Catalog No.	AP1507
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62071

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S616 of human IRS1(NP_005535.1).
Sequence	PMSPGV
Gene ID
Swiss Prot
Synonyms	HIRS-1; Phospho-IRS1-S612/S636
Calculated MW	132kDa
Observed MW	180kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	C2C12 treated by Insulin
Cellular location	Caveola, Cytoplasm, Cytosol, Nucleoplasm, Nucleus, Plasma membrane
Customer validation	WB(Homo sapiens)