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Phospho-IRS1-S312 Rabbit pAb (AP0181)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293 cells, using Phospho-IRS1-S312 Rabbit pAb (AP0181).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-IRS1-S312 Rabbit pAb
Catalog No.	AP0181
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Immunogen information

Immunogen	A phospho specific peptide corresponding to residues surrounding S312 of human IRS1
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	HIRS-1; Phospho-IRS1-S312
Calculated MW	132kDa
Observed MW	180kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293
Cellular location	caveola, cytoplasm, cytosol, nucleoplasm, nucleus, plasma membrane
Customer validation	WB(Homo sapiens, Mus musculus)