제품 > 항체 > 인산화 특이적 항체

Phospho-IRS1-S612 Rabbit pAb (AP0553)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from C2C12 cells using Phospho-IRS1-S612 Rabbit pAb (AP0553) at 1:1000 dilution. C2C12 cells were treated by Insulin (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Western blot analysis of lysates from MCF7 cells using Phospho-IRS1-S612 Rabbit pAb (AP0553) at 1:1000 dilution. MCF7 cells were treated by Insulin (100 nM) at 37℃ for 30 minutes after serum starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-IRS1-S612 Rabbit pAb (AP0553)

Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-IRS1-S612 Rabbit pAb (AP0553) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-IRS1-S612 Rabbit pAb (AP0553) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-IRS1-S612 Rabbit pAb (AP0553) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product name	Phospho-IRS1-S612 Rabbit pAb
Catalog No.	AP0553
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S612 of human IRS1 (NP_005535.1).
Sequence	PMSPG
Gene ID
Swiss Prot
Synonyms	HIRS-1; Phospho-IRS1-S612
Calculated MW	132kDa
Observed MW	180kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	C2C12, MCF7
Cellular location	caveola, cytoplasm, cytosol, nucleoplasm, nucleus, plasma membrane
Customer validation	WB(Homo sapiens, Mus musculus)