제품 > 항체 > 인산화 특이적 항체

Phospho-PAK2-S20 Rabbit mAb (AP1443)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Phospho-PAK2-S20 Rabbit mAb (AP1443)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PAK2-S20 Rabbit mAb (AP1443) at 1:2000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-PAK2-S20 Rabbit mAb (AP1443)

Western blot analysis of lysates from C6 cells, using Phospho-PAK2-S20 Rabbit mAb (AP1443) at 1:2000 dilution.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product namePhospho-PAK2-S20 Rabbit mAb
Catalog No.AP1443
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62567
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
ImmunogenA synthetic phosphorylated peptide around S20 of human PAK2 (NP_002568.2).
SequenceMSSTI
Gene ID
Swiss Prot
SynonymsKNO2; PAK65; PAKgamma; Phospho-PAK2-S20
Calculated MW58kDa
Observed MW61kDa/67kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by Calyculin A, C6 treated by Calyculin A
Cellular locationCytoplasm, Membrane, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Phospho-PAK2-S20 Rabbit mAb (AP1443)}

Western blot - Phospho-PAK2-S20 Rabbit mAb (AP1443)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PAK2-S20 Rabbit mAb (AP1443) at 1:2000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Phospho-PAK2-S20 Rabbit mAb (AP1443)}

Western blot - Phospho-PAK2-S20 Rabbit mAb (AP1443)

Western blot analysis of lysates from C6 cells, using Phospho-PAK2-S20 Rabbit mAb (AP1443) at 1:2000 dilution.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (4)

Secondary Antibodies (26)