Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from 293T cells, using Phospho-PERK-T982 Rabbit mAb (AP1501) at 1:1000 dilution.293T cells were treated by Insulin (100 nM) at 37℃ for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Phospho-PERK-T982 Rabbit mAb (AP1501) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-PERK-T982 Rabbit mAb (AP1501) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Phospho-PERK-T982 Rabbit mAb (AP1501) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Phospho-PERK-T982 Rabbit mAb (AP1501) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-PERK-T982 Rabbit mAb (AP1501) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
| Product name | Phospho-PERK-T982 Rabbit mAb |
|---|---|
| Catalog No. | AP1501 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC58934 |
| Immunogen | A synthetic phosphorylated peptide around T982 of human PERK(NP_004827.4). |
|---|---|
| Sequence | RHTGQV |
| Gene ID | |
| Swiss Prot | |
| Synonyms | PEK; WRS; PERK; Phospho-PERK-T982 |
| Calculated MW | 125kDa |
| Observed MW | 170kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | 293T treated by Insulin |
| Cellular location | Endoplasmic reticulum membrane |
| Customer validation | WB(Mus musculus, Cavia porcellus) IHC(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using AP1501? Please let us know so that we can cite the reference in this datasheet.
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