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Phospho-PKCδ/θ-S643/676 Rabbit pAb (AP0567)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from RAW264.7 cells, using Phospho-PKCδ/θ-S643/676 Rabbit pAb (AP0567) at 1:1000 dilution. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes .
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name	Phospho-PKCδ/θ-S643/676 Rabbit pAb
Catalog No.	AP0567
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S643 of human PKCδ/θPRKCD (NP_001341605.1).
Sequence	RLSYS
Gene ID
Swiss Prot
Synonyms	PRKCD/PRKCQ; Phospho-PKCδ/θ-S643/676
Calculated MW	77kDa/80kDa/67kDa/74kDa/81kDa
Observed MW	78kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	RAW264.7 treated by PMA
Cellular location	cell-cell junction, cytoplasm, cytosol, endolysosome, endoplasmic reticulum, extracellular exosome, extracellular region, mitochondrion, nuclear matrix, nucleoplasm, nucleus, perinuclear region of cytoplasm, plasma membrane