제품 > 항체 > 인산화 특이적 항체

Phospho-PKC mu-S744/748 Rabbit pAb (AP0534)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PKC mu-S744/748 pAb (AP0534) at 1:1000 dilution or PRKD1 antibody (A16185). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name	Phospho-PKC mu-S744/748 Rabbit pAb
Catalog No.	AP0534
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S744 & S748 of mouse PKC mu (NP_032884.2).
Sequence	EKSFRRS
Gene ID
Swiss Prot
Synonyms	PKD; PKCM; CHDED; PRKCM; PKC-MU; Phospho-PKC mu-S744/748
Calculated MW	102kDa
Observed MW	102kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by PMA
Cellular location	Cell membrane, Cytoplasm, Golgi apparatus, trans-Golgi network.