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Phospho-PKC (pan) (βII Ser660) Rabbit pAb (AP0495)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-PKC (pan) (βII Ser660) Rabbit pAb (AP0495)

Western blot analysis of lysates from 293T cells, using Phospho-PKC (pan) (βII Ser660) pAb (AP0495) at 1:1000 dilution or PRKCQ antibody (A5834). 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product namePhospho-PKC (pan) (βII Ser660) Rabbit pAb
Catalog No.AP0495
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic phosphorylated peptide around S660 of human PKC (pan) βIIPRKCA (NP_002728.2).
SequenceYVNPQ
Gene ID
Swiss Prot
SynonymsPRKCA/PRKCB/PRKCD/PRKCE/PRKCH/PRKCQ; Phospho-PKC (pan) (βII Ser660)
Calculated MW59kDa/67kDa/74- 83kDa
Observed MW95kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by PMA
Cellular locationcytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, mitochondrial membrane, mitochondrion, nucleoplasm, perinuclear region of cytoplasm, plasma membrane.

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    ABclonal:Western blot - Phospho-PKC (pan) (βII Ser660) Rabbit pAb (AP0495)}

    Western blot - Phospho-PKC (pan) (βII Ser660) Rabbit pAb (AP0495)

    Western blot analysis of lysates from 293T cells, using Phospho-PKC (pan) (βII Ser660) pAb (AP0495) at 1:1000 dilution or PRKCQ antibody (A5834). 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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