제품 > 항체 > 인산화 특이적 항체

Phospho-PLN-S16 Rabbit pAb (AP0907)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-PLN-S16 Rabbit pAb (AP0907) at 1:1000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from 293F cells using Phospho-PLN-S16 Rabbit pAb (AP0907) at 1:1000 dilution incubated overnight at 4℃. 293F cells transfected with PLN were treated by λ-PP mixed solution (1μL) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	Phospho-PLN-S16 Rabbit pAb
Catalog No.	AP0907
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S16 of human PLN (NP_002658.1).
Sequence	RASTI
Gene ID
Swiss Prot
Synonyms	PLB; CMD1P; CMH18; Phospho-PLN-S16
Calculated MW	6kDa
Observed MW	12KkDa/17kDa/35kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C2C12 cells and C6 cells were treated by CIP, 293F cells transfected with PLN were treated by λ-PP
Cellular location	endoplasmic reticulum, mitochondrial membrane, mitochondrion, perinuclear region of cytoplasm.
Customer validation	WB(Rattus norvegicus)