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Phospho-PRKCQ-S676 Rabbit pAb (AP0260)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-PRKCQ-S676 Rabbit pAb (AP0260)

Western blot analysis of lysates from Jurkat cells, using Phospho-PRKCQ-S676 Rabbit pAb (AP0260) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product namePhospho-PRKCQ-S676 Rabbit pAb
Catalog No.AP0260
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
ImmunogenA phospho specific peptide corresponding to residues surrounding S676 of human PRKCQ
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsPRKCT; nPKC-theta; Phospho-PRKCQ-S676
Calculated MW82kDa
Observed MW78kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat treated by PMA
Cellular locationCell membrane, Cytoplasm, Peripheral membrane protein.

Documents

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ABclonal:Western blot - Phospho-PRKCQ-S676 Rabbit pAb (AP0260)}

Western blot - Phospho-PRKCQ-S676 Rabbit pAb (AP0260)

Western blot analysis of lysates from Jurkat cells, using Phospho-PRKCQ-S676 Rabbit pAb (AP0260) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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