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Phospho-RB-S807/811 Rabbit pAb (AP0484)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HT-29 cells, using Phospho-RB-S807/811 Rabbit pAb (AP0484) at 1:1000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product name	Phospho-RB-S807/811 Rabbit pAb
Catalog No.	AP0484
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S807 & S811 of human RB (NP_000312.2).
Sequence	SPLKS
Gene ID
Swiss Prot
Synonyms	RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1; Phospho-RB-S807/811
Calculated MW	106kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HT-29 treated by Nocodazole
Cellular location	Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus)