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Phospho-Rac1/Cdc42-S71 Rabbit mAb (AP1018)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of extracts of A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb (AP1018) at 1:1000 dilution.A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product name	Phospho-Rac1/Cdc42-S71 Rabbit mAb
Catalog No.	AP1018
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1563

Background

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S71 of human Rac1/Cdc42CDC42 (P60953).
Sequence	PLSYP
Gene ID
Swiss Prot
Synonyms	CDC42Hs; G25K; TKS
Calculated MW	24kDa
Observed MW	24kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	A-431
Cellular location	Cytoplasm, Cytoplasmic ribonucleoprotein granule, Cytosol, dendritic spine, Extracellular exosome, Filopodium, Focal adhesion, Golgi transport complex, Leading edge membrane, Microtubule organizing center, Mitotic spindle, Phagocytic vesicle, Plasma membrane, Spindle midzone
Customer validation	WB(Homo sapiens,Rattus norvegicus)