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Phospho-Ret-Y905 Rabbit pAb (AP0600)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-Ret-Y905 Rabbit pAb (AP0600)

Western blot analysis of extracts of K562 cells, using Phospho-Ret-Y905 antibody (AP0600).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-Ret-Y905 Rabbit pAb
Catalog No.AP0600
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.
ImmunogenA synthetic phosphorylated peptide around Y905 of human Ret (NP_066124.1).
SequenceDSYVK
Gene ID
Swiss Prot
SynonymsPTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
Calculated MW124kDa
Observed MW124kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesK-562
Cellular locationCell membrane, Endosome membrane, Single-pass type I membrane protein
Customer validation

ELISA(Mus musculus)

WB(Mus musculus)

ABclonal:Western blot - Phospho-Ret-Y905 Rabbit pAb (AP0600)}

Western blot - Phospho-Ret-Y905 Rabbit pAb (AP0600)

Western blot analysis of extracts of K562 cells, using Phospho-Ret-Y905 antibody (AP0600).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)