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Ret Rabbit pAb (A0018)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Ret Rabbit pAb (A0018)

Western blot analysis of lysates from Rat brain, using Ret Rabbit pAb (A0018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - Ret Rabbit pAb (A0018)

Immunofluorescence analysis of Neuro-2a cells using Ret Rabbit pAb (A0018) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Ret Rabbit pAb (A0018)

Immunofluorescence analysis of SH-SY5Y cells using Ret Rabbit pAb (A0018) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Ret Rabbit pAb(A0018)

Overview

Product nameRet Rabbit pAb
Catalog No.A0018
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human Ret (NP_066124.1).
SequenceDISKDLEKMMVKRRDYLDLAASTPSDSLIYDDGLSEEETPLVDCNNAPLPRALPSTWIENKLYGMSDPNWPGESPVPLTRADGTNTGFPRYPNDSVYANWM
Gene ID
Swiss Prot
SynonymsPTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1; Ret
Calculated MW124kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationCell membrane, Endosome membrane, Single-pass type I membrane protein
Customer validation

(293T)

WB(Homo sapiens)

Documents

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ABclonal:Western blot - Ret Rabbit pAb (A0018)}

Western blot - Ret Rabbit pAb (A0018)

Western blot analysis of lysates from Rat brain, using Ret Rabbit pAb (A0018) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - Ret Rabbit pAb (A0018)}

Immunofluorescence - Ret Rabbit pAb (A0018)

Immunofluorescence analysis of Neuro-2a cells using Ret Rabbit pAb (A0018) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Ret Rabbit pAb (A0018)}

Immunofluorescence - Ret Rabbit pAb (A0018)

Immunofluorescence analysis of SH-SY5Y cells using Ret Rabbit pAb (A0018) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)