Western blot - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Western blot analysis of lysates from 293T cells, using Phospho-SMC1-S957 Rabbit mAb (AP1145) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SMC1-S957 Rabbit mAb (AP1145) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Immunohistochemistry - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-SMC1-S957 Rabbit mAb (AP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Phospho-SMC1-S957 Rabbit mAb (AP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Phospho-SMC1-S957 Rabbit mAb (AP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Phospho-SMC1-S957 Rabbit mAb (AP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-SMC1-S957 Rabbit mAb (AP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-SMC1-S957 Rabbit mAb (AP1145)
Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Phospho-SMC1-S957 Rabbit mAb (AP1145) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.