Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of lysates from RAW 264.7 cells using Phospho-STING-S365 Rabbit pAb (AP1199) at 1:1000 dilution. RAW264.7 cells were transfection with poly(dA:dT) (5ug/mL) for 3 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
| Product name | Phospho-STING/TMEM173-S365 Rabbit pAb |
|---|---|
| Catalog No. | AP1199 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S365 of mouse STING (NP_082537.1). |
|---|---|
| Sequence | LISGMD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ERIS; MPYS; Mita; STING; Tmem173; STING-beta; 2610307O08Rik; Phospho-STING-S365 |
| Calculated MW | 43kDa |
| Observed MW | 41kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | RAW 264.7 |
| Cellular location | autophagosome, cytoplasm, cytosol, endoplasmic reticulum, endosome, Golgi apparatus, mitochondrial outer membrane, nucleoplasm, perinuclear region of cytoplasm, peroxisome, plasma membrane. |
| Customer validation | WB(Homo sapiens, Mus musculus, Mus musculus) |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using AP1199? Please let us know so that we can cite the reference in this datasheet.
