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Phospho-SnRK2.6-S175 pAb (AP1481)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Arabidopsis thaliana

Western blot analysis of lysates from recombinant Phospho-SnRK2.6-S175 Protein using Phospho-SnRK2.6-S175 pAb (AP1481) at 1:500 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 100 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 0.5 s.

Overview

Product name	Phospho-SnRK2.6-S175 pAb
Catalog No.	AP1481
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Activator of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomata closure in response to drought, darkness, high CO2, plant pathogens, or decreases in atmospheric relative humidity (RH) (PubMed:30361234).

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S175 of Arabidopsis thaliana SnRK2.6(NP_567945.1).
Sequence	PKS（p）TV
Gene ID
Swiss Prot
Synonyms	SnRK2.6; SRK2E
Calculated MW	38kDa/41kDa
Observed MW	83kDa

Applications

Reactivity	Arabidopsis thaliana
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Recombinat Phospho-SnRK2.6-S175 Protein
Cellular location	Nucleus.