제품 > 항체 > 인산화 특이적 항체

Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (AP0501)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb (AP0501) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product name	Phospho-Syk-Y352/ZAP70-Y319 Rabbit pAb
Catalog No.	AP0501
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y352 of human Syk (NP_003168.2).
Sequence	SPYAD
Gene ID
Swiss Prot
Synonyms	SYK/ZAP70; Phospho-Syk-Y352/ZAP70-Y319
Calculated MW	69kDa/72kDa/35kDa/55kDa
Observed MW	69kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Jurkat, Jurkat treated by 10% FBS
Cellular location	cytoplasm, cytosol, early phagosome, nucleus, plasma membrane.