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Phospho-TBC1D4-T642 Rabbit pAb (AP0791)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Phospho-TBC1D4-T642 Rabbit pAb (AP0791)

Western blot analysis of lysates from 293T cells, using Phospho-TBC1D4-T642 Rabbit pAb (AP0791) at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/mL) for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product namePhospho-TBC1D4-T642 Rabbit pAb
Catalog No.AP0791
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic phosphorylated peptide around T642 of human TBC1D4 (NP_055647.2).
SequenceAHTFS
Gene ID
Swiss Prot
SynonymsAS160; NIDDM5; Phospho-TBC1D4-T642
Calculated MW147kDa
Observed MW160kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by IGF-1
Cellular locationCytoplasm
ABclonal:Western blot - Phospho-TBC1D4-T642 Rabbit pAb (AP0791)}

Western blot - Phospho-TBC1D4-T642 Rabbit pAb (AP0791)

Western blot analysis of lysates from 293T cells, using Phospho-TBC1D4-T642 Rabbit pAb (AP0791) at 1:2000 dilution. 293T cells were treated by IGF-1 (50ng/mL) for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)