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Phospho-TP73-Y99 Rabbit pAb (AP0465)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-TP73-Y99 Rabbit pAb (AP0465)

Western blot analysis of lysates from MCF7 cells, using Phospho-TP73-Y99 Rabbit pAb (AP0465) at 1:500 dilution. MCF7 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product namePhospho-TP73-Y99 Rabbit pAb
Catalog No.AP0465
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y99 of human TP73
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsP73; CILD47; Phospho-TP73-Y99
Calculated MW70kDa
Observed MW80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7 treated by pervanadate
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-TP73-Y99 Rabbit pAb (AP0465)}

Western blot - Phospho-TP73-Y99 Rabbit pAb (AP0465)

Western blot analysis of lysates from MCF7 cells, using Phospho-TP73-Y99 Rabbit pAb (AP0465) at 1:500 dilution. MCF7 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

* For research use only. Not for therapeutic or diagnostic purposes.

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