제품 > 항체 > 다중클론항체(pAb)

TP73 Rabbit pAb (A0385)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - TP73 Rabbit pAb (A0385)

Western blot analysis of lysates from 293T cells, using TP73 Rabbit pAb (A0385) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - TP73 Rabbit pAb (A0385)

Immunohistochemistry analysis of paraffin-embedded human lymph node using TP73 Rabbit pAb (A0385) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TP73 Rabbit pAb (A0385)

Immunohistochemistry analysis of paraffin-embedded human esophageal using TP73 Rabbit pAb (A0385) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TP73 Rabbit pAb (A0385)

Immunofluorescence analysis of paraffin-embedded mouse skin using TP73 Rabbit pAb (A0385) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameTP73 Rabbit pAb
Catalog No.A0385
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 487-636 of human TP73 (NP_005418.1).
SequenceYHADPSLVSFLTGLGCPNCIEYFTSQGLQSIYHLQNLTIEDLGALKIPEQYRMTIWRGLQDLKQGHDYSTAQQLLRSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGPGGGPDEWADFGFDLPDCKARKQPIKEEFTEAEIH
Gene ID
Swiss Prot
SynonymsP73; CILD47; TP73
Calculated MW70kDa
Observed MW80kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - TP73 Rabbit pAb (A0385)}

Western blot - TP73 Rabbit pAb (A0385)

Western blot analysis of lysates from 293T cells, using TP73 Rabbit pAb (A0385) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - TP73 Rabbit pAb (A0385)}

Immunohistochemistry - TP73 Rabbit pAb (A0385)

Immunohistochemistry analysis of paraffin-embedded human lymph node using TP73 Rabbit pAb (A0385) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TP73 Rabbit pAb (A0385)}

Immunohistochemistry - TP73 Rabbit pAb (A0385)

Immunohistochemistry analysis of paraffin-embedded human esophageal using TP73 Rabbit pAb (A0385) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TP73 Rabbit pAb (A0385)}

Immunofluorescence - TP73 Rabbit pAb (A0385)

Immunofluorescence analysis of paraffin-embedded mouse skin using TP73 Rabbit pAb (A0385) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0385? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)