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Phospho-Tyk2-Y1054/1055 Rabbit pAb (AP0543)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Tyk2-Y1054/1055 Rabbit pAb (AP0543) at 1:1000 dilution. NIH/3T3 cells were treated by IFN-α (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product name	Phospho-Tyk2-Y1054/1055 Rabbit pAb
Catalog No.	AP0543
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the tyrosine kinase and, more specifically, the Janus kinases (JAKs) protein families. This protein associates with the cytoplasmic domain of type I and type II cytokine receptors and promulgate cytokine signals by phosphorylating receptor subunits. It is also a component of both the type I and type III interferon signaling pathways. As such, it may play a role in anti-viral immunity. A mutation in this gene has been associated with Immunodeficiency 35.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y1054 & Y1055 of human Tyk2 (NP_003322.3).
Sequence	HEYYRV
Gene ID
Swiss Prot
Synonyms	JTK1; IMD35; Phospho-Tyk2-Y1054/1055
Calculated MW	134kDa
Observed MW	134kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by IFN-α
Cellular location	cytoplasm, cytoskeleton, cytosol, extracellular exosome, nucleus.
Customer validation	WB(Mus musculus, Homo sapiens)