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Phospho-ULK1-S555 Rabbit mAb (AP1495)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 293T cells using Phospho-ULK1-S555 Rabbit mAb (AP1495) at 1:8000 dilution incubated overnight at 4℃. 293T cells were treated by λ-PP mixed solution (62.5U) at 30℃ for 1 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunofluorescence - Phospho-ULK1-S555 Rabbit mAb (AP1495)

Confocal imaging of C2C12 cells (treated with CA) and C2C12 cells (untreated) using Phospho-ULK1-S555 Rabbit mAb (AP1495, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name	Phospho-ULK1-S555 Rabbit mAb
Catalog No.	AP1495
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63423

Background

Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S555 of human ULK1 (NP_003556.2).
Sequence	LHS(P)AP
Gene ID
Swiss Prot
Synonyms	ATG1; ATG1A; UNC51; hATG1; Unc51.1
Calculated MW	113kDa
Observed MW	140-150KD

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:9000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	239T, 239T+λpp
Cellular location	Cytoplasm, preautophagosomal structure, cytosol.