제품 > 항체 > 다중클론항체(pAb)

ULK1 Rabbit pAb (A8529)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Rat brain, using ULK1 Rabbit pAb (A8529) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Western blot analysis of various lysates, using ULK1 Rabbit pAb (A8529) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - ULK1 Rabbit pAb (A8529)

Immunofluorescence analysis of HeLa cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ULK1 Rabbit pAb (A8529) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	ULK1 Rabbit pAb
Catalog No.	A8529
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human ULK1 (NP_003556.1).
Sequence	SSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQS
Gene ID
Swiss Prot
Synonyms	ATG1; ATG1A; UNC51; hATG1; Unc51.1; ULK1
Calculated MW	113kDa
Observed MW	150kDa/140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T, HepG2, Rat brain, Mouse thymus, Rat testis
Cellular location	Cytoplasm, Preautophagosomal structure, cytosol
Customer validation	WB(Homo sapiens, Mus musculus, Spodoptera frugiperda, Sus scrofa, Fathead minnow, Horabagrus nigricollaris, Gallus gallus) IHC(Mus musculus) IHC(Ctenopharyngodon idellus) WB(Ctenopharyngodon idellus)

Documents

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Research Area

ULK1 Rabbit pAb images

Western blot - ULK1 Rabbit pAb (A8529)

Immunofluorescence - ULK1 Rabbit pAb (A8529)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Oncology letters

Application:

IF:

2.967

Species:

Homo sapiens

PMID:

29928348

Title:

Activation of the SphK1/ERK/p-ERK pathway promotes autophagy in colon cancer cells

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

International Immunopharmacology

Application:

IF:

4.932

Species:

Mus musculus

PMID:

31655340

Title:

Macrophages-derived p38α promotes the experimental severe acute pancreatitis by regulating inflammation and autophagy

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Virulence

Application:

IF:

5.882

Species:

Spodoptera frugiperda

PMID:

33509041

Title:

Autophagy induced by Vip3Aa has a pro-survival role in Spodoptera frugiperda Sf9 cells

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Brain Res Bull

Application:

IF:

4.077

Species:

Mus musculus

PMID:

32871241

Title:

Myocardial infarction-induced anxiety-like behavior is associated with epigenetic alterations in the hippocampus of rat

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Sus scrofa

PMID:

36035212

Title:

Biogenic Selenium Nanoparticles Alleviate Intestinal Epithelial Barrier Damage through Regulating Endoplasmic Reticulum Stress-Mediated Mitophagy - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Cellular signalling

Application:

IF:

4.85

Species:

Homo sapiens

PMID:

36270475

Title:

FOXO3a-dependent up-regulation of HSP90 alleviates cisplatin-induced apoptosis by activating FUNDC1-mediated mitophagy in hypoxic osteosarcoma cells

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Journal of Cancer

Application:

IF:

4.47

Species:

Homo sapiens

PMID:

36186900

Title:

Snake venom induces an autophagic cell death via activation of the JNK pathway in colorectal cancer cells - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Journal of oncology

Application:

IF:

4.5

Species:

Homo sapiens

PMID:

36590307

Title:

Bee Venom Triggers Autophagy-Induced Apoptosis in Human Lung Cancer Cells via the mTOR Signaling Pathway - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

International journal of molecular medicine

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

37026504

Title:

Dihydroartemisinin inhibits the activation and proliferation of hepatic stellate cells by regulating miR-29b-3p - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Cancer cell international

Application:

IF:

6.42

Species:

Homo sapiens

PMID:

37587444

Title:

A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Immunity, inflammation and disease

Application:

IF:

2.49

Species:

Mus musculus

PMID:

37904715

Title:

Naringenin ameliorates collagen-induced arthritis through activating AMPK-mediated autophagy in macrophages - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Immunity, inflammation and disease

Application:

IF:

2.49

Species:

Mus musculus

PMID:

37904715

Title:

Naringenin ameliorates collagen-induced arthritis through activating AMPK-mediated autophagy in macrophages - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Fish & shellfish immunology

Application:

IF:

4.62

Species:

Fathead minnow

PMID:

37661036

Title:

Chlorpyrifos-mediated mitochondrial calcium overload induces EPC cell apoptosis via ROS/AMPK/ULK1

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.988

Species:

Homo sapiens

PMID:

35140613

Title:

Deciphering the Mechanism of Bushen Huoxue Decotion on Decidualization by Intervening Autophagy via AMPK/mTOR/ULK1: A Novel Discovery for URSA Treatment

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

The Science of the total environment

Application:

IF:

10.75

Species:

Homo sapiens

PMID:

37951264

Title:

FUNDC1-mediated mitophagy triggered by mitochondrial ROS is partially involved in 1-nitropyrene-evoked placental progesterone synthesis inhibition and

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Fish & shellfish immunology

Application:

IF:

4.7

Species:

Homo sapiens

PMID:

38365163

Title:

miR-193b-5p promotes GCRV replication by inhibiting autophagy via targeting deptor in grass carp (Ctenopharyngodon idellus)

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Oral diseases

Application:

IF:

3.8

Species:

Mus musculus

PMID:

38321366

Title:

Sodium fluorideinduced autophagy of ameloblastlike cells via the pULk1/ATG13/LC3B pathway in vitro

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Bioengineered

Application:

WB, IHC

IF:

6.832

Species:

Mus musculus

PMID:

35435132

Title:

miR-129-5p targets FEZ1/SCOC/ULK1/NBR1 complex to restore neuronal function in mice with post-stroke depression

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Horabagrus nigricollaris

PMID:

36902147

Title:

Dietary Leucine Improves Fish Intestinal Barrier Function by Increasing Humoral Immunity, Antioxidant Capacity, and Tight Junction - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

International journal of biological sciences

Application:

IF:

9.2

Species:

Homo sapiens

PMID:

38617533

Title:

IL-33 Accelerates Chronic Atrophic Gastritis through AMPK-ULK1 Axis Mediated Autolysosomal Degradation of GKN1 - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IF:

Species:

Gallus gallus

PMID:

38840220

Title:

Hypoxia-mediated programmed cell death is involved in the formation of wooden breast in broilers - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Toxicology

Application:

IF:

4.5

Species:

Mus musculus

PMID:

38710382

Title:

ROS-mediated NRF2/p-ERK1/2 signaling-involved mitophagy contributes to macrophages activation induced by CdTe quantum dots

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

BMC cancer

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

39061024

Title:

MARCH1 negatively regulates TBK1-mTOR signaling pathway by ubiquitinating TBK1 - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Biochimica et biophysica acta. Molecular cell research

Application:

IF:

5.1

Species:

Mus musculus

PMID:

37597773

Title:

Exogenous H2S alleviates senescence of glomerular mesangial cells through up-regulating mitophagy by activation of AMPK-ULK1-PINK1-parkin pathway in mice

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IHC, WB

IF:

Species:

Ctenopharyngodon idellus

PMID:

39218924

Title:

Methionine deficiency inhibited pyroptosis in primary hepatocytes of grass carp (Ctenopharyngodon idella): possibly via activating the ROS-AMPK-autophagy axis - PMC

References for ULK1 Rabbit pAb (A8529)

Product:

ULK1 Rabbit pAb

Journal:

Autophagy

Application:

IF:

13.3

Species:

Sus scrofa

PMID:

38516932