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Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368)

Western blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368) at 1:400 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) at 37℃ for 6 hours after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product namePhospho-Ubiquitin-Ser65 Rabbit pAb
Catalog No.AP1368
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
ImmunogenA synthetic phosphorylated peptide around S65 of human Ubiquitin (NP_002945.1).
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsUBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80; L40; CEP52; RPL40; HUBCEP52; HEL-S-50; HMG20; Phospho-Ubiquitin-Ser65
Calculated MW18kDa/15kDa/77kDa
Observed MW10-250kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-3 treated by Carbonyl cyanide 3-chlorophenylhydrazone
Cellular locationCytoplasm, Nucleus.

Documents

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    ABclonal:Western blot - Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368)}

    Western blot - Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368)

    Western blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368) at 1:400 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) at 37℃ for 6 hours after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)