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Phospho-VAV1-Y174 Rabbit pAb (AP0180)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Phospho-VAV1-Y174 Rabbit pAb (AP0180)

Western blot analysis of lysates from Jurkat cells, using Phospho-VAV1-Y174 Rabbit pAb (AP0180).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Overview

Product namePhospho-VAV1-Y174 Rabbit pAb
Catalog No.AP0180
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding Y174 of human VAV1
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsVAV; Phospho-VAV1-Y174
Calculated MW98kDa
Observed MW98kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat
Cellular locationcell-cell junction, cytoplasm, cytosol, plasma membrane
ABclonal:Western blot - Phospho-VAV1-Y174 Rabbit pAb (AP0180)}

Western blot - Phospho-VAV1-Y174 Rabbit pAb (AP0180)

Western blot analysis of lysates from Jurkat cells, using Phospho-VAV1-Y174 Rabbit pAb (AP0180).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)