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Phospho-ZAP70-Y493 Rabbit mAb (AP1435)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - Phospho-ZAP70-Y493 Rabbit mAb (AP1435)

Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat using Phospho-ZAP70-Y493 Rabbit mAb (AP1435) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePhospho-ZAP70-Y493 Rabbit mAb
Catalog No.AP1435
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59392
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around Y493 of human ZAP70.
SequenceSYYTA
Gene ID
Swiss Prot
SynonymsZAP70; ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70; tyrosine-protein kinase ZAP-70
Calculated MW70kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    
Positive samples
Cellular locationCell membrane, Cytoplasm, Peripheral membrane protein

Documents

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ABclonal:Immunofluorescence - Phospho-ZAP70-Y493 Rabbit mAb (AP1435)}

Immunofluorescence - Phospho-ZAP70-Y493 Rabbit mAb (AP1435)

Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat using Phospho-ZAP70-Y493 Rabbit mAb (AP1435) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (5)

Secondary Antibodies (26)