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Phospho-c-Myc-S62 Rabbit mAb (AP0989)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using Phospho-c-Myc-S62 Rabbit mAb (AP0989) at 1:1000 dilution. 293T cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	Phospho-c-Myc-S62 Rabbit mAb
Catalog No.	AP0989
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1533

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S62 of human c-Myc (NP_002458.2).
Sequence	PLSPS
Gene ID
Swiss Prot
Synonyms	MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-S62
Calculated MW	51kDa
Observed MW	62kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by Forskolin, NIH/3T3 treated by Forskolin
Cellular location	Nucleus, nucleolus, nucleoplasm.
Customer validation	WB(Homo sapiens, Mus musculus) WB (Mus musculus)