Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from 293T cells, using Phospho-eEF2-T56 Rabbit pAb (AP0832) at 1:3000 dilution. 293T cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
| Product name | Phospho-eEF2-T56 Rabbit pAb |
|---|---|
| Catalog No. | AP0832 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around T56 of human Phospho-eEF2-T56 (NP_001952.1). |
|---|---|
| Sequence | TRFTD |
| Gene ID | |
| Swiss Prot | |
| Synonyms | EF2; EF-2; EEF-2; SCA26; Phospho-eEF2-T56 |
| Calculated MW | 95kDa |
| Observed MW | 95kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T treated by FBS |
| Cellular location | Cytoplasm, Nucleus. |
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* For research use only. Not for therapeutic or diagnostic purposes.
_WB_01.jpg?t=1718355589 "ABclonal:Western blot - Phospho-eEF2-T56 Rabbit pAb (AP0832)")
