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eEF2 Rabbit mAb (A9721)

Review (1)Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - eEF2 Rabbit mAb (A9721)

Western blot analysis of various lysates using eEF2 Rabbit mAb (A9721) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - eEF2 Rabbit mAb (A9721)

Western blot analysis of lysates from Rat spleen, using eEF2 Rabbit mAb (A9721) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - eEF2 Rabbit mAb (A9721)

Immunohistochemistry analysis of paraffin-embedded Human appendix using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - eEF2 Rabbit mAb (A9721)

Immunofluorescence analysis of C6 cells using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - eEF2 Rabbit mAb (A9721)

Immunofluorescence analysis of NIH-3T3 cells using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - eEF2 Rabbit mAb (A9721)

Immunofluorescence analysis of U-2 OS cells using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for eEF2 Rabbit mAb(A9721)

Overview

Product nameeEF2 Rabbit mAb
Catalog No.A9721
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1717
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 759-858 of human eEF2 (P13639).
SequenceIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL
Gene ID
Swiss Prot
SynonymsEF2; EF-2; EEF-2; SCA26; eEF2
Calculated MW95kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:100 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, Jurkat, Mouse liver, Rat spleen
Cellular locationCytoplasm, Nucleus.
Customer validation

(293T,Hela)

WB(Homo sapiens)

RIP(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - eEF2 Rabbit mAb (A9721)}

Western blot - eEF2 Rabbit mAb (A9721)

Western blot analysis of various lysates using eEF2 Rabbit mAb (A9721) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - eEF2 Rabbit mAb (A9721)}

Western blot - eEF2 Rabbit mAb (A9721)

Western blot analysis of lysates from Rat spleen, using eEF2 Rabbit mAb (A9721) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - eEF2 Rabbit mAb (A9721)}

Immunohistochemistry - eEF2 Rabbit mAb (A9721)

Immunohistochemistry analysis of paraffin-embedded Human appendix using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - eEF2 Rabbit mAb (A9721)}

Immunofluorescence - eEF2 Rabbit mAb (A9721)

Immunofluorescence analysis of C6 cells using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - eEF2 Rabbit mAb (A9721)}

Immunofluorescence - eEF2 Rabbit mAb (A9721)

Immunofluorescence analysis of NIH-3T3 cells using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - eEF2 Rabbit mAb (A9721)}

Immunofluorescence - eEF2 Rabbit mAb (A9721)

Immunofluorescence analysis of U-2 OS cells using eEF2 Rabbit mAb (A9721) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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