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Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363)

Western blot analysis of lysates from 293T cells, using Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363) at 1:20000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363) at 1:20000 dilution. NIH/3T3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product namePhospho-eIF4EBP1-S65 Rabbit mAb
Catalog No.AP1363
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53775
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
ImmunogenA synthetic phosphorylated peptide around S65 of human Phospho-EIF4EBP1-S65 (NP_004086.1).
SequenceRNSPV
Gene ID
Swiss Prot
SynonymsBP-1; 4EBP1; 4E-BP1; PHAS-I; Phospho-eIF4EBP1-S65
Calculated MW13kDa
Observed MW15-20kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by IGF-1, NIH/3T3
Cellular locationcytoplasm, cytosol.
Customer validation

WB(Homo sapiens, Mus musculus, Bos taurus)

Other(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363)}

Western blot - Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363)

Western blot analysis of lysates from 293T cells, using Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363) at 1:20000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363)}

Western blot - Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eIF4EBP1-S65 Rabbit mAb (AP1363) at 1:20000 dilution. NIH/3T3 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (11)

Secondary Antibodies (26)