제품 > 항체 > 다중클론항체(pAb)

eIF4EBP1 Rabbit pAb (A1248)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using eIF4EBP1 Rabbit pAb (A1248) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	eIF4EBP1 Rabbit pAb
Catalog No.	A1248
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 50-118 of human eIF4EBP1 (NP_004086.1).
Sequence	TRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI
Gene ID
Swiss Prot
Synonyms	BP-1; 4EBP1; 4E-BP1; PHAS-I; eIF4EBP1
Calculated MW	13kDa
Observed MW	15-20kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:100 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	MCF7, HeLa, Mouse skeletal muscle
Cellular location	cytoplasm, cytosol.
Customer validation	WB(Macaca mulatta, Homo sapiens, Mus musculus, Bos taurus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

eIF4EBP1 Rabbit pAb images

Western blot - eIF4EBP1 Rabbit pAb (A1248)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Journal Of Proteomics

Application:

IF:

4.044

Species:

Macaca mulatta

PMID:

26361011

Title:

iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Frontiers in molecular neuroscience

Application:

IF:

5.639

Species:

Homo sapiens

PMID:

28348518

Title:

Combination of Endothelial-Monocyte-Activating Polypeptide-II with Temozolomide Suppress Malignant Biological Behaviors of Human Glioblastoma Stem Cells via miR-590-3p/MACC1 Inhibiting PI3K/AKT/mTOR Signal Pathway

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Nature communications

Application:

IF:

14.919

Species:

Mus musculus

PMID:

28541289

Title:

miR-29b contributes to multiple types of muscle atrophy

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Stem Cell Res Ther

Application:

IF:

6.832

Species:

Homo sapiens

PMID:

33579362

Title:

Alpha-enolase (ENO1), identified as an antigen to monoclonal antibody 12C7, promotes the self-renewal and malignant phenotype of lung cancer stem cells by AMPK/mTOR pathway

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Mol Ther Nucleic Acids

Application:

IF:

8.886

Species:

Mus musculus

PMID:

33614226

Title:

Angiotensin II-induced muscle atrophy via PPARγ suppression is mediated by miR-29b

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

J Sport Health Sci

Application:

IF:

7.179

Species:

Mus musculus

PMID:

34116237

Title:

Exercise attenuates angiotensinⅡ-induced muscle atrophy by targeting PPARγ/miR-29b

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Biochem Pharmacol

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

34111425

Title:

Inhibition of Pim-2 kinase by LT-171-861 promotes DNA damage and exhibits enhanced lethal effects with PARP Inhibitor in multiple myeloma

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Molecular therapy : the journal of the American Society of Gene Therapy

Application:

IF:

12.91

Species:

Mus musculus

PMID:

33279721

Title:

Inhibition of lncRNA MAAT controls multiple types of muscle atrophy by cis-and trans-regulatory actions

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Frontiers in aging neuroscience

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

36589543

Title:

The identities of insulin signaling pathway are affected by overexpression of Tau and its phosphorylation form - PMC

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Food and chemical toxicology

Application:

IF:

5.572

Species:

Homo sapiens

PMID:

30578841

Title:

Corynoxine B ameliorates HMGB1-dependent autophagy dysfunction during manganese exposure in SH-SY5Y human neuroblastoma cells.

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

eLife

Application:

IF:

8.71

Species:

Homo sapiens,Mus musculus

PMID:

35975983

Title:

Biphasic regulation of osteoblast development via the ERK MAPK-mTOR pathway - PMC

References for eIF4EBP1 Rabbit pAb (A1248)

Product:

eIF4EBP1 Rabbit pAb

Journal:

Animals : an open access journal from MDPI

Application:

IF:

Species:

Bos taurus

PMID:

38200771

Title:

Glucose Regulates Glucose Transport and Metabolism via mTOR Signaling Pathway in Bovine Placental Trophoblast Cells

Publishing research using A1248? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)

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제품 > 항체 > 다중클론항체(pAb)

eIF4EBP1 Rabbit pAb (A1248)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

eIF4EBP1 Rabbit pAb images

Product Protocols

항체 (11)

Secondary Antibodies (26)