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Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)

Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)

Western blot analysis of lysates from C6 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 20 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-p38 MAPK-T180/Y182 Rabbit mAb
Catalog No.AP1502
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62666
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
ImmunogenA synthetic phosphorylated peptide around T180/Y182 of human MAPK(NP_620581.1).
SequenceMTGYVA
Gene ID
Swiss Prot
SynonymsRK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; Phospho-p38 MAPK-T180/Y182
Calculated MW41kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by UV, NIH/3T3 treated by UV, C6 treated by Anisomycin
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)}

    Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)

    Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)}

    Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)

    Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)}

    Western blot - Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502)

    Western blot analysis of lysates from C6 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (AP1502) at 1:1000 dilution. C6 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 20 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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