제품 > 항체 > 다중클론항체(pAb)

Pqbp1 Rabbit pAb (A18680)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Pqbp1 Rabbit pAb (A18680)

Western blot analysis of lysates from Mouse brain, using Pqbp1 Rabbit pAb (A18680) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - Pqbp1 Rabbit pAb (A18680)

Western blot analysis of lysates from Rat brain, using Pqbp1 Rabbit pAb (A18680) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product namePqbp1 Rabbit pAb
Catalog No.A18680
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable double-stranded DNA binding activity; protein C-terminus binding activity; and ribonucleoprotein complex binding activity. Involved in alternative mRNA splicing, via spliceosome and neuron projection development. Acts upstream of or within regulation of dendrite morphogenesis. Located in cytoplasmic stress granule; neuronal ribonucleoprotein granule; and nuclear speck. Is expressed in several structures, including 1st branchial arch maxillary component; adrenal cortex; central nervous system; eye; and limb. Used to study Renpenning syndrome. Human ortholog(s) of this gene implicated in Renpenning syndrome. Orthologous to human PQBP1 (polyglutamine binding protein 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 170-263 of mouse Pqbp1 (NP_062351.2).
SequenceEGKDRRHHRREELAPYPKNKKATSRKDEELDPMDPSSYSDAPRGTWSTGLPKRNEAKTGADTTAAGPLFQQRPYPSPGAVLRANAEASRTKQQD
Gene ID
Swiss Prot
SynonymsSfc2; npw38; PQBP-1; Pqbp1
Calculated MW31kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Rat brain
Cellular locationcentrosome, ciliary base, cilium, cytoplasm, cytoplasmic stress granule, neuronal ribonucleoprotein granule, nuclear body, nuclear speck, nucleus
    ABclonal:Western blot - Pqbp1 Rabbit pAb (A18680)}

    Western blot - Pqbp1 Rabbit pAb (A18680)

    Western blot analysis of lysates from Mouse brain, using Pqbp1 Rabbit pAb (A18680) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Western blot - Pqbp1 Rabbit pAb (A18680)}

    Western blot - Pqbp1 Rabbit pAb (A18680)

    Western blot analysis of lysates from Rat brain, using Pqbp1 Rabbit pAb (A18680) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 3min.

    * For research use only. Not for therapeutic or diagnostic purposes.