제품 > 항체 > 다중클론항체(pAb)

Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Western blot analysis of various lysates using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Immunohistochemistry analysis of paraffin-embedded Human benign prostatic hyperplasia using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Immunohistochemistry analysis of paraffin-embedded human kidney using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Immunohistochemistry analysis of paraffin-embedded human prostate cancer using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameProstatic Acid Phosphatase (ACPP) Rabbit pAb
Catalog No.A1937
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 33-386 of human PAP/Prostatic Acid Phosphatase (ACPP) (NP_001090.2).
SequenceKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQGTEDSTD
Gene ID
Swiss Prot
SynonymsACPP; 5'-NT; ACP-3; TM-PAP; Prostatic Acid Phosphatase (ACPP)
Calculated MW45kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse thymus, Rat liver, Rat thymus
Cellular locationCell membrane, Lysosome membrane, Secreted, Single-pass type I membrane protein

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)}

Western blot - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Western blot analysis of various lysates using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)}

Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Immunohistochemistry analysis of paraffin-embedded Human benign prostatic hyperplasia using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)}

Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Immunohistochemistry analysis of paraffin-embedded human kidney using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)}

Immunohistochemistry - Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937)

Immunohistochemistry analysis of paraffin-embedded human prostate cancer using Prostatic Acid Phosphatase (ACPP) Rabbit pAb (A1937) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)