제품 > 항체 > 단일클론항체(mAb)

Pumilio 2 Rabbit mAb (A19783)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Pumilio 2 Rabbit mAb (A19783)

Western blot analysis of lysates from Rat testis, using Pumilio 2 Rabbit mAb (A19783) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Pumilio 2 Rabbit mAb (A19783)

Western blot analysis of various lysates using Pumilio 2 Rabbit mAb (A19783) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product namePumilio 2 Rabbit mAb
Catalog No.A19783
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2308
This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Pumilio 2 (Q8TB72).
SequenceMNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRSGQGFHGNSEVNAILSPRSESGGLGVSMVEYVLS
Gene ID
Swiss Prot
SynonymsPUMH2; PUML2; Pumilio 2
Calculated MW114kDa
Observed MW110-125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-3, Mouse testis
Cellular locationCytoplasm, Cytoplasmic stress granule, Cytosol, Nuclear membrane, Perinuclear region of cytoplasm

Documents

Certificate of Compliance

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ABclonal:Western blot - Pumilio 2 Rabbit mAb (A19783)}

Western blot - Pumilio 2 Rabbit mAb (A19783)

Western blot analysis of lysates from Rat testis, using Pumilio 2 Rabbit mAb (A19783) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Pumilio 2 Rabbit mAb (A19783)}

Western blot - Pumilio 2 Rabbit mAb (A19783)

Western blot analysis of various lysates using Pumilio 2 Rabbit mAb (A19783) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.